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Anti-NMDA Receptor, NR1 Subunit, C1 Splice Variant Insert
Colorado Biosciences Park
12635 East Montview Boulevard, #213
Aurora, CO 80045-7337
Tel: (888) 442-7100

Page 1 of 2

WB = Western Blot IF = Immunofluorescence IHC = Immunohistochemistry IP = Immunoprecipitation
Packaging: 25
g antibody vial; lyophilized from 5 mM ammonium bicarbonate. The antibody should be reconstituted in 50 µl phosphate
buffered saline (PBS: 137 mM NaCl, 7.5 mM Na
2
HPO
4
, 2.7 mM KCl, 1.5 mM KH
2
PO
4
, pH 7.4) before use. After reconstitution the antibody
should be aliquoted and stored
at -20
o
C.
Adequate amount of material to conduct 10-mini Western Blots.
Storage and Stability: For long term storage 20
o
C is recommended. Stable at 20
o
C for at least 1 year.
Shipment: Domestic - Ambient; International Ambient.

Anti-NMDA Receptor, NR1 Subunit, C1 Splice Variant Insert

Catalog Number: 1505-C1
Size: 25 µg
$295.00

Product Description: Affinity purified rabbit polyclonal antibody

Applications:
WB: 1:1000
IHC
(frozen sections; unpublished observations): 1:1000 to 1:2000

Antigen: Peptide from the NR1 subunit, C1 splice variant insert of the NMDA receptor.

Species reactivity: The antibody has been directly tested for reactivity in Western blots in rat
and mouse tissues. It is anticipated that the antibody will also work with bovine, canine, human,
and non-human primate tissues based on the fact that these species have 100% homology with
the amino acid sequence used as antigen.

Biological Significance: The ion channels activated by glutamate that are sensitive to N-methyl-D-
aspartate (NMDA) are designated NMDA Receptors (NMDAR). The NMDAR plays an essential role in
memory and neuronal development and it has also been implicated in several disorders of the central
nervous system including Alzheimers, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002;
Wenthold et al., 2003; Carroll and Zukin, 2002). There are a number of different splice variants of the
NR1-Subunit (Foldes et al., 1994; Zukin and Bennett, 1995). Differential splicing of three exons in the
NR1-Subunit generates up to eight NR1-Subunit splice variants and 7 of these have been identified in
cDNA libraries. These exons encode a 21 amino acid N-terminal domain (N1) and adjacent sequences in
the C-terminus (C1 and C2). Splicing out the C2 cassette eliminates the first stop codon and produces a
new reading frame that generates a new sequence of 22 amino acids (C2'). Considerable attention has
been focused on the distribution and expression of these splice variants that may affect the functional
properties and regulation of the NMDAR.
Anti-NMDAR, NR1 Subunit, C1 Splice Variant Insert

Western blot
of 10 ug of HEK 293 cells expressing: Lane 1 - HEK cells without NR1 expression
(Mock); Lane 2 - NR1 subunit containing only the C2 Insert; Lane 3 - NR1 subunit containing the
C1 and C2' Insert; Lane 4 - NR1 subunit containing the N1 and C2' Insert showing specific
immunolabeling of the ~120k NR1 subunit of the NMDA receptor containing the C1 splice variant
insert.
Page 2 of 2

WB = Western Blot IF = Immunofluorescence IHC = Immunohistochemistry IP = Immunoprecipitation
Packaging: 25
g antibody vial; lyophilized from 5 mM ammonium bicarbonate. The antibody should be reconstituted in 50 µl phosphate
buffered saline (PBS: 137 mM NaCl, 7.5 mM Na
2
HPO
4
, 2.7 mM KCl, 1.5 mM KH
2
PO
4
, pH 7.4) before use. After reconstitution the antibody
should be aliquoted and stored
at -20
o
C.
Adequate amount of material to conduct 10-mini Western Blots.
Storage and Stability: For long term storage 20
o
C is recommended. Stable at 20
o
C for at least 1 year.
Shipment: Domestic - Ambient; International Ambient.

Purification Method: Prepared from rabbit serum by affinity purification using a column to
which the peptide immunogen was coupled.

Antibody Specificity: Specific for the ~120k NR1 subunit of the NMDA receptor containing the
C1 splice variant insert. Does not recognize the NR1 subunits of the NMDA receptor that do not
contain the C1 insert.

Quality Control Tests: Western blots performed on each lot.

References:

Carroll RC, Zukin RS (2002) NMDA-receptor trafficking and targeting: implications for synaptic transmission and
plasticity. Trends Neurosci 25:571-577.
Foldes RL, Rampersad V, Kamboj RK (1994) Cloning and sequence analysis of additional splice variants encoding
human N-methyl-D-aspartate receptor (hNR1) subunits. Gene 147:303-304.
Grosshans DR, Clayton DA, Coultrap SJ, Browning MD (2002) LTP leads to rapid surface expression of NMDA but
not AMPA receptors in adult rat CA1. Nat Neurosci 5:27-33.
Wenthold RJ, Prybylowski K, Standley S, Sans N, Petralia RS (2003) Trafficking of NMDA receptors. Annu Rev
Pharmacol Toxicol 43:335-358.
Zukin RS, Bennett MVL (1995) Alternatively spliced isoforms of the NMDARI receptor subunit. Trends Neurosci
18:306-313.

Note: Dr. Michael Browning, a co-author of one of the cited papers, is President and founder of PhosphoSolutions.