Combination Scattering Meter and Fluorometer
r>This users guide is an evolving document. If you find sections that are unclear or missing
information, please let us know. Please check our website periodically for updates.
WET Labs, Inc.
PO Box 518
Philomath, OR 97370
(541) 929-5650
www.wetlabs.com
ECO BB2F Users Guide (bb2f)
Revision AA
11 September 2007
Attention!
Return Policy for Instruments with Anti-fouling Treatment
WET Labs cannot accept instruments for servicing or repair that are treated with anti-
fouling compound(s). This includes but is not limited to tri-butyl tin (TBT), marine anti-
fouling paint, ablative coatings, etc.
Please ensure any anti-fouling treatment has been removed prior to returning instruments
to WET Labs for service or repair.
ECO BB2F Users Guide (bb2f)
Revision AA
11 September 2007
ECO Sensor Warranty
This unit is guaranteed against defects in materials and workmanship for one year from the
original date of purchase. Warranty is void if the factory determines the unit was subjected to
abuse or neglect beyond the normal wear and tear of field deployment, or in the event the
pressure housing has been opened by the customer.
To return the instrument, contact WET Labs for a Return Merchandise Authorization (RMA) and
ship in the original container. WET Labs is not responsible for damage to instruments during the
return shipment to the factory. WET Labs will supply all replacement parts and labor and pay for
return via 3
rd
day air shipping in honoring this warranty.
Shipping Requirements
1. Please retain the original Pelican® shipping case. It meets stringent shipping and insurance
requirements, and protects your meter.
2. Service and repair work cannot be guaranteed unless the meter is shipped in its original case.
3. Clearly mark the RMA number on the outside of your case and on all packing lists.
4. Return instruments using 3
rd
day air shipping or better: do not ship via ground.
ECO BB2F Users Guide (bb2f)
Revision AA
11 September 2007
ECO BB2F Users Guide (bb2f)
Revision AA
11 September 2007
i
Table of Contents
1.
Overview ...................................................................................... 1
1.1
Specifications..................................................................................................... 1
1.2
Connectors ......................................................................................................... 2
1.3
Optional Equipment ........................................................................................... 3
1.4
Delivered Items.................................................................................................. 5
2.
Theory of Operation ..................................................................... 7
3.
Instrument Operation.................................................................... 9
3.1
Initial Checkout.................................................................................................. 9
3.2
Operating the Sensor for Data Output ............................................................... 9
3.3
Deployment...................................................................................................... 10
3.4
Upkeep and Maintenance................................................................................. 10
4.
BB2FB: Using Internal Batteries................................................. 13
4.1
Removing End Flange and Batteries................................................................ 13
4.2
Replacing End Flange and Batteries ................................................................ 14
4.3
Checking Vent Plug ......................................................................................... 15
5.
Data Analysis ............................................................................. 16
5.1
Scattering Data Corrections ............................................................................. 16
5.2
Derived Parameters.......................................................................................... 17
6.
Testing and Calibration/Characterization ................................... 19
6.1
Testing.............................................................................................................. 19
6.2
Scattering Calibration ...................................................................................... 19
6.3
Fluorometer Characterization .......................................................................... 20
7.
Terminal Communications......................................................... 21
7.1
Interface Specifications.................................................................................... 21
7.2
Command List.................................................................................................. 21
8.
Device and Output Files ............................................................ 22
8.1
Plot Header....................................................................................................... 22
8.2
Column Count Specification............................................................................ 22
8.3
Column Description......................................................................................... 22
8.4
Sample Device and Output File Formats ......................................................... 23
Appendix A: Mounting Bracket Drawing ........................................... 24
1. Overview
1.1
Specifications
Model BB2F
BB2FB
Mechanical
Diameter
6.3 cm
6.3 cm
Length
12.7 cm
25.4 cm
Weight in air
0.4 kg
0.96 kg
Weight in water
0.02 kg
0.14 kg
Material
Acetal copolymer
Acetal copolymer
Environmental
Temperature range
030 deg C
030 deg C
Depth rating
600 m
300 m
Pressure/temperature
sensor (optional)
Yes
Yes
Electrical
Digital output resolution
12 bit
12 bit
Internal data logging
Yes Yes
Internal batteries
No Yes
Connector
MCBH6M MCBH6M
Input
715 VDC
715 VDC
Current, typical
80 mA
80 mA
Current, sleep
85 µA
85 µA
Data memory
50,000 samples
50,000 samples
Sample rate
to 8 Hz
to 8 Hz
RS-232 output
19200 baud
19200 baud
Optical
Scattering wavelengths
470 and 700 nm
470 and 700 nm
Sensitivity, blue
2.4 x 10
-5
m
-1
sr
-1
2.4 x 10
-5
m
-1
sr
-1
Sensitivity, red
3.35 x 10
-6
m
-1
sr
-1
3.35 x 10
-6
m
-1
sr
-1
Range, typical
~ 0.00245 m
-1
~ 0.00245 m
-1
Fluorometer EX/EM
470/695 nm
470/695 nm
Sensitivity
0.02 µg/l
0.02 µg/l
Range, typical
0.0268 µg/l
0.0268 µg/l
Linearity
99% R
2
99% R
2
ECO BB2F Users Guide (bb2f)
Revision AA
11 September 2007
1
1.2
Connectors
The ECO BB2F uses a six-pin bulkhead connector; the pin functions for this connector are
shown in Figure 1. Table 1 summarizes pin functions for the bulkhead connectors.
Figure 1. ECO-BB2F connector schematic
Table 1. Pinout summary for ECO BB2F connectors
Pin (or Socket)
Function
1 Ground
2 RS-232
(RX)
3 Reserved
4 V
in
5 RS-232
(TX)
6 Reserved
WARNING
If you are going to build or use a non-WET Labs cable, do not use the wire from
pin 3 or the ECO meter will be damaged.
Input power of 715 VDC is applied to pin 4. The power supply current returns through the
common ground pin. The input power signal has a bi-directional filter. This prevents external
power supply noise from entering into ECO-BB2F, and also prevents internally generated
noise from coupling out on to the external power supply wire. Data is sent out the serial output
pin.
1.2.1 ECO BB2FB Connectors
The ECO BB2FB (unit with internal batteries) has a second bulkhead connector that comes
with a jumper plug to supply power to the unit. The pin functions for this connector are
shown in Figure 2. Table 2 summarizes pin functions for the 3-socket bulkhead connector.
2 ECO BB2F Users Guide (bb2f)
Revision AA
11 September 2007
Figure 2
.
ECO BB2FB connector schematic
Table 2. Pinout summary for ECO 3-socket connector
Pin (or Socket)
Function
1 V
in
2 N/C
3 Battery
out
1.3
Optional Equipment
1.3.1 Test
Cable
A test cable is optionally supplied with each unit. This cable includes three legs:
1. A connector for providing power to the instrument from a user-supplied 9V battery.
2. A DB-9 serial interface connector.
3. A six-socket in-line connector for providing power and signal to the instrument.
1.3.2 Copper
Faceplate
ECO meters are optionally equipped with copper faceplates to improve the meters
resistance to biofouling. Refer to Section 3.4.1 for important details on maintenance and
cleaning.
ECO BB2F Users Guide (bb2f)
Revision AA
11 September 2007
3
1.3.3 Batteries
ECO units with internal batteries are supplied with six 9-volt Lithium batteries as their
power source. They can use either standard alkaline cells for a total capacity of
approximately 1000 mA-hrs, or for longer deployments, LiMnO
2
cells to achieve more than
2000 mA-hrs of operational capacity. Actual total usage time of the internal batteries is a
function of several parameters. These include nominal water temperature, sequence timing,
sample periods, and total deployment duration.
WARN