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Tecnai G
P R O D U C T

D A T A
Understanding
decreasing dimensions
Tecnai G
2
Spirit TEM
Next generation microscopy
for life sciences
The stringent demands of life science and soft matter
research require t and adequate microscopy techniques
for imaging and analysis. The Tecnai G
2
Spirit is designed
to meet this purpose with a high level of performance.
The microscope is offered in two, patented lens
congurations: the BioTWIN for high contrast imaging or
the TWIN for general high resolution purposes. The Spirit
produces excellent 2D and 3D images with automated
functionality featured in a seamless embedded manner.
The Tecnai G
2
Spirit TWIN, a 20 to 120 kV / LaB
6
transmission
electron microscope (TEM), is a general purpose, high
resolution instrument. It enables the 2D and 3D ultra-
structure exploration of cells and cell constituents, as well as
the morphology, chemical composition and function of
natural or articial materials that play a crucial role in modern
science and technology. Low dose observation of beam
sensitive and cryo samples is a key performance aspect.
The Tecnai G
2
Spirit BioTWIN, a 20 to 120 kV / LaB
6

transmission electron microscope, is a high contrast, general
purpose instrument that can perform the same tasks and
analysis as the TWIN version, but is specically suited for low
contrast samples. It enables exploration of the native state of
low contrast, beam sensitive biological specimens, or other
soft materials like polymers. Samples can be stained or
unstained and the lens design guarantees maximum contrast,
even at low magnications.
The microscope has embedded automation - an option that
users can choose at will. The gun or illumination can be
tuned, aligned, saturated and conditioned automatically
or manually. The same exibility holds for focus and
stigmation. Automation is fast and convenient and lowers
the threshold for novice users and helps in securing quality
of results.
Key benefits
High contrast and high resolution for 20 to 120 kV operation
Optimized for 2D & 3D imaging of cells, cell organelles
and soft matter
High level of automation: Auto-Gun and automatic tuning
Smart tracking position system for sample navigation
Customized protocols for diverse applications
Sample observation at liquid nitrogen temperature
Technology for sharp imaging of thicker samples
Ergonomic design for operational comfort
Outstanding analytical performance
RAPID enabled for remote diagnostics P R O D U C T

D A T A
Electron source
W or LaB
6
emitter
Auto-saturation
Auto-conditioning
LaB
6
lifetime > 1 year
Filament change < 5 min.
High voltage range 20 to 120 kV or
continuously variable high tension change < 1 min.
Illumination system
Four lenses
User selectable intensity limit for specimen protection
User selectable zoom for constant screen intensity
Imaging
Five lens magnification system
High contrast, long focal length objective lens (BioTWIN)
Automated contrast enhancement function
Focusing aid, adjustable wobbler for all magnifications
and directions
Focus preset for focusing at eucentric position
Rotation free magnification and diffraction series
Magnification aberration corrected lens series
Embedded CCD and TV camera*
Plate camera with 56 sheets of film*
Low dose software*

Micro analysis*
Embedded STEM and EDX
Small probes (< 0.3 nm)
- No spurious / system peaks
- STEM magnification: 150 to 3.1 Mx
- Resolution: 1.0 nm
Specimen stage
Fully computer controlled, eucentric side entry,
high stability CompuStage
High tilt and large field of view
Choice of a variety of specimen holders,
(cryo, multiple, rotation holders etc.)
X, Y movement 2 mm, specimen size 3 mm
Specimen position store and recall including
magnification and illumination settings
0.5 µm (x, y) and 0.5° (tilt)
Drift < 1 nm/min with standard holder
Specimen exchange pumping time 10 to 180 sec.
(user adjustable)
Specimen exchange without switching off high tension
and emitter
High tilt (± 80°) and large field of view

*optional
Essential specifications
Objective lens
TWIN
BioTWIN
Line resolution (nm)
0.20
0.34
C
s
objective (mm)
2.2
6.3
C
c
objective (mm)
2.2
5.0
Focal length (mm)
2.8
6.1
Minimum focus step (nm)
3.0
9.0
Maximum eucentric tilt
± 70°
± 80°
Magnification
22 to 740 000
22 to 340 000
Specimen exchange time
< 30 seconds
< 30 seconds
High tension switching time
< 1 minute
< 1 minute P R O D U C T

D A T A
Vacuum
Fully interlocked, differentially pumped column
Ultra-high vacuum for contamination free observation of
the specimen
TWIN: cryo box* (not EDX compatible)
BioTWIN: fixed cryo shields standard (EDX compatible)
Vacuum levels: specimen chamber and gun area 10
-6
Pa
Plate camera exchange without switching off high tension
and emitter
Operation / automation
Operating system: Windows
®
XP
New ergonomic hand panels
Remote operation*
Scripting SW module*
2
nd
data monitor*
3D imaging using Xplore3D
TM
intelligent tomography
solution*
Upgradeable functionality by uploading current and future
application software solutions

*optional
Cable lengths:
ABCD=500
EF=245
Electronic
rack
Microscope
console
C
D
F
A
E
B
min. 45
min
. 15
0
min. 250
m
i
n
.

3
0
0
min. 30
min. 30
min. 60
Measurements in cm
Floorplan WORLD HEADQUARTERS
5350 NE DAWSON CREEK DRIVE
HILLSBORO, OREGON 97124 USA
PH: +1.503.726.7500

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PH: +81.3.3740.0970

FEI ASIA PACIFIC
PH: +86.21.6122.5988
fei.com
©2008. We are constantly improving the performance of our products,
so all specifications are subject to change without notice. The FEI logo, Tecnai,
Xplore3D and Tools for Nanotech are trademarks of FEI Company.
02DS-TE0113 04/08
TÜV Certification for design, manufacture,
installation and support of focused ion-
and electron-beam microscopes for
the NanoElectronics, NanoBiology,
NanoResearch and Industry markets.
P R O D U C T

D A T A
Figure 1: Negative stain preparation of rota virus. Sample courtesy of
Cynthia Goldsmith, Center for Disease Control, Atlanta, USA.
Figure 3: Cow pea mosaic virus sample frozen with Vitrobot.
Figure 2: Nerve biopsy from a patient with a peripheral neuropathy.
Sample courtesy of Dr. Wayne Moore and Ms. Susan Shinn, University of British
Columbia, Vancouver, Canada.
Figure 4: 3D model of multilamellar lysosomes in B-lymphocytes.
Courtesy of Dr. Jean-Luc Murk, Department of Cell Biology, Utrecht University,
The Netherlands.